The aberrant expression of ROR2 in melanoma promotes the expression of endothelial cell markers.

MASCOLO P; QUEZADA MJ; BARRAZA, JESUS; LOPEZ BERGAMI P

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias 2022; 2022

Sociedad Argentina de Investigacion Clinica

Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a membrane receptor of the Wnt5a ligand and a mayor component of the Wnt non-canonical pathway. ROR2 was shown to play a dual role in cancer by either suppressing or promoting tumor progression in different tumor types. Recent findings from our group have established that ROR2 can play this dual role simultaneously in melanoma cell lines and has been proposed to be a main regulator of melanoma cell plasticity.The aim of this work is to further characterize the complex role of ROR2 in melanoma. To investigate the biological and biochemical mechanisms regulated by ROR2, both gain- and loss-of-function approaches were employed by generating A375 cell lines with both overexpression and silencing of ROR2. Overexpression of ROR2 significantly increased the expression levels of the endothelial cell markers PECAM, PDGF-R, VCAM1, and Tie2 (P