BECAS
CHOP Maia
congresos y reuniones científicas
Título:
HYDATID FLUID FROM ECHINOCOCCUS GRANULOSUS IS RECOGNIZED BY CLEC9A IN BMDCs, INDUCES AUTOPHAGY AND POLARIZES THE T CELL RESPONSE TOWARDS TH-1/TH-17 PROFILES IN VITRO.
Autor/es:
CHOP, MAIA; LEDO CAMILA; NICOLAO, MARÍA CELESTE; LOOS, JULIA; CUMINO, ANDREA C.; RODRIGUEZ RODRIGUES, CHRISTIAN
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Anual de Sociedades de Biociencias (SAIC-SAI-SAFIS); 2022
Institución organizadora:
SAIC, SAI, SAFIS
Resumen:
Background: Cystic echinococcosis is caused by Echinococcus granulosus. This parasite develops cysts in the host that are filled with hydatid fluid (HF). Autophagy plays a key role in antigen presentation promoting activation of T cells. The aim of this work is to analyze if HF could polarize the T cell response in vitro. Methods: HF was collected from infected cattle. Expression of Clec9a was evaluated by FACS in FLT3L-BMDCs. Autophagy induction was evaluated by FACS, qPCR, confocal and TEM. Rapamycin (20 nM) and chloroquine (100μM) were used to analyze autophagic flux. T cell cytokine profiles were analyzed by qPCR. Results: HF induces a downmodulation of Clec9a in BMDCs (n=3 **p
