Effects of oligonucleotide IMT504 in a metabolic syndrome and type 2 diabetes model induced by high-fat diet in mice.

BONAVENTURA MM; DOMÉ L; MONTANER A; LUX LANTOS VAR; BIANCHI MS

Mar del plata

Congreso; Reunión Anual de Sociedades de Biociencias: LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXX Reunión Anual de la Sociedad Argentina de Inmunología (SAI) & 3rd French-Argentine Immunology Congress (FAIC), Reunión Anual 2022; 2022

SAIC

OLIGONUCLEOTIDE IMT504 IMPROVES METABOLIC PARAMETERS AND FOOD INTAKE IN A METABOLIC SINDROME AND TYPE 2 DIABETES MODEL INDUCED BY HIGH-FAT DIET IN MICEEFFECTS OF OLIGONUCLEOTIDE IMT504 IN A METABOLIC SINDROME AND TYPE 2 DIABETES MODEL INDUCED BY HIGH-FAT DIET IN MICE USO ESTE TÍTULOMaría Marta Bonaventura1, Laila Domé1, Alejandro Montaner2, Victoria Adela Lux-Lantos1, María Silvia Bianchi11Instituto de Biología y Medicina Experimental (CONICET), Buenos Aires, Argentina.2Instituto de Ciencia y Tecnología “Dr. Cesar Milstein” (CONICET – Fundación P. Cassará), Buenos Aires, Argentina.We have shown that the immunomodulatory oligonucleotide IMT504 improves glucose homeostasis in animal models of type I diabetes by regulating expression of immune modulatory factors and by improving beta cell function. Here we evaluated the effects of IMT504 in a murine model of metabolic syndrome and type 2 diabetes induced by a high-fat diet.Male C57BL/6LP mice were fed either a standard diet (SD) or a high-fat diet (HFD: ResearchDiet, D12492) for 12 weeks. HFD animals showed higher non-fasting glycemia (Gly: p˂0.01), and body weight (BW: p˂0.01). Mice received one daily dose of IMT504 for 12 consecutive days (IMT: 20mg/kg/day, 6mg/kg/day or 2mg/kg/day) or saline. Glucose tolerance tests (GTT, day 10) and insulin tolerance tests (ITT, day 11) were performed. On day 12, food intake, BW, and non-fasted Gly were recorded; after 3 hours fasting, mice were sacrificed and blood samples collected. Gly did not vary with time in SD and HFD mice, while it significantly diminished with IMT treatment [Gly (mg/dl): repeated measures ANOVA: interaction, p