In-vitro effects of BPA, BP2 and BP3 on cell proliferation in mature GnRH neurons

RIAÑO GÓMEZ JUAN; SORIANELLO, E; LUX LANTOS VAR; FERNANDEZ MO

Glasgow

Congreso; International Congress of Neuroendocrinology; 2022

International Congress of Neuroendocrinology

In-vitro effects of BPA, BP2 and BP3 on cell proliferation in mature GnRH neuronsRiaño Gómez JM1, Sorianello EM1, Lux-Lantos VAR1 and Fernandez MO1. 1-Instituto de Biología y Medicina Experimental-CONICET, Email: juanrianog@gmail.comPreviously we showed that the in-vitro exposure to BPA, BP2 and BP3, endocrine disruptors, (ED, 1x10-7 and 1x10-9 M, 24 hs) increased cell proliferation in an immature GnRH cell line, GN11 cells (Susan Wray, USA). Kisspeptin also has a proliferative effect on these cells. The aim of this study is to evaluate the effects of the in-vitro exposure of the aforementioned compounds on cell proliferation in GT1-7 cells (mature GnRH neurons, Pamela Mellon, UCSD, USA). Cell proliferation was evaluated using a Non-Radioactive Cell Proliferation Assay, MTS (Promega, WI, USA), after BPA, E2, BP2 and BP3 exposure (1x10-7 and 1x10-9 M, 12 or 24 hs). Participation of the nuclear estrogen receptors was evaluated using the estrogen receptor antagonist ICI 182780, (1x10-6 M). Effects of Kisspeptin (Kiss, 1x10-9 M) on cell proliferation was also evaluated. Results were recorded as Abs490/Abs490(Control), presented as Mean±SE and analyzed by Repeated measures ANOVA with a Fisher posttest (Statistica, StatSoft, OK, USA). Neither BPA nor BP2 modified cell proliferation (ANOVA ns, n=5). BP3 on the other hand increased cell proliferation compared to control values both after 12 and 24 h exposure [12h-exposure: Control=1±0.08, BP3-9=1.27±0.12, BP3-7=1.39±0.13, BP2-9=1,04±0,05, BP2-7=1,01±0,06, BP3-7 different from Control p