. Effect of anagrelide on megakaryocytic cytoskeleton during platelet production. possible genes involved.

CELLUCCI AS; LEV PR; YAÑUK D; GOETTE N; GLEMBOTSKY AC; DE LUCA G; LIRA C; BARONI PIETTO C; HELLER PG; MARTA RF

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2022

We previously reported changes in maximum platelet diameter (MPD) in patients with Phi-negative chronic myeloproliferative neoplasms treated with different drugs. Anagrelide (ANA), alpha Interferon (IFN) and ruxolitinib (Ruxo) increased MPD, while hydroxyurea (HU) decreased MPD. Evaluation of proplatelet (PP) morphology in normal megakaryocyte (MK) culture showed that ANA and IFN increased the size of tips and swellings (structures that give rise to platelets), while Ruxo and HU did not.To deepen into possible causes of these changes during ANA treatment, two approaches were carried outA) Morphological analysis of MK cytoskeleton by indirect immunofluorescence: a trend toward decreased actin polarization was observed (phalloidin-FITC), suggesting that demarcation membrane development necessary for thrombopoiesis is abnormal. Additionally, microtubular disorganization was observed (anti-alpha tubulin-FITC) (Wilcoxon test p