SYNERGISTIC ANTI-Trypanosoma Cruzi EFFECTIVENESS OF CANDIMINE COMBINED WITH BENZNIDAZOLE BY THE CHOU-TALALAY METHOD

PIÑEIRO M.; MARTINEZ-PEINADO N; ORTIZ J.E..; BARRERA P; SOSA MA; ALONSO-PADILLA J; FERESIN GE.

Mendoza

Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2022

Sociedad de Biologia de Cuyo

Chagas disease, caused by the parasite T. cruzi, affects over six million people worldwide, mainly in Latin American countries. Currently available drugs, Nifurtimox or Benznidazol (Bzn), have variable efficacy in the chronic phase and significant side effects. Recently, evidence has emerged in favor of the use of drug combinations to improve the efficacy and tolerance of treatment. Amaryllidaceae plants have proved to be a potential natural source of therapeutic agents due to their rich diversity in alkaloids. The Hippeastrum genus is endemic to South America. Candimine (Cnd), isolated from H. morelianum, reported activity against the protozoan Trichomonas vaginalis. The aim of this study was to evaluate a the combined activity between Cnd and Bzn against T. cruzi by means of the Chou-Talalay method, additionally its cytotoxicity profile was determined against two mammalian cell lines (Vero and HepG2) to ascertain its selectivity against the parasite and potential liver toxicity. The Combination Index (CI) and the Dose Reduction Index (DRI) were estimated using CompuSyn software. Compounds were combined in a fixed dose ratio corresponding to 0.25; 0.5; 1; 2 and 4 times that of the individual IC50 values. In epimastigotes, the interaction was synergistic (IC