In vitro evaluation of sodium hyaluronate protective effect against benzalkonium chloride toxicity

DEL PAPA, MELINA; SABBIONE, FLORENCIA; VEREERTBRUGGHEN, ALEXIA; PASSERINI, MARÍA SILVIA; GALLETTI JEREMÍAS GASTÓN

Denver, CO

Congreso; ARVO 2022 Annual Meeting; 2022

Association for Research in Vision & Ophthalmology

Purpose : Long-term exposure to benzalkonium chloride (BAK) causes toxicity reactions on the ocular surface. Sodium hyaluronate (SH) has been postulated as a potential neutralizing agent for BAK-induced toxicity. The goal of this work was to evaluate the protective effect of SH on BAK-induced toxicity using an in vitro model.Methods : The NAV14 cell line (SV40-immortalized murine conjunctival epithelium) was used. Cell monolayers were exposed to different concentrations of BAK (0.001%; 0.005%; 0.01%) and SH (0.2%; 0.3%; 0.4%) for 15 minutes; then, the cells were washed, and fresh culture media was added. Cell viability was evaluated after 2 h by resazurin reduction and lactate dehydrogenase (LDH) enzyme release. Also, cell migration and proliferation over 24 hours were determined by the scratch wound-healing assay. Data was analyzed by two-way ANOVA and is shown as mean±SD of two independent experiments with 4-6 replicates each. Statistical significance was defined as p