Effects On Bone Disease Of Mechanical Stimulation By Whole Body Vibration In A Murine Model of CKD. Preliminary Study

PÉREZ BETIANA MABEL; SCHRECK C; PEREZ PJ; BONANNO MARINA SOLEDAD; DIEHL MARÍA; ZENI, SUSANA NOEMÍ; MANDALUNIS PATRICIA; PLANTALECH LUISA; ROSA-DIEZ GUILLERMO

Austin

Congreso; ASMBR 2022 Annual Meeting; 2022

The American Society for Bone and Mineral Research (ASBMR)

Background: Mechanical stimulation by treadmill running in rodents with chronic kidney disease (CKD) lowers sclerostin (Scl) and improves spine and femoral metaphysis BMD. We hypothesized that mechanical stimulation by whole body vibration (WBV) in a CKD model could limit bone disease, reducing Scl production. The objective of this study was to evaluate bone effects of WBV in an experimental model of CKD.Methods: Wistar male rats aged 13 wk old were randomly assigned to: A) Control (n 5), B) Control and whole body vibration (CWBV) (n 6), C) CKD (n 5), D) CKD and WBV (n 5). Resection of 80% of renal mass was performed to groups C and D; sham surgery to A and B. Two weeks after surgery groups B and D were placed on a low intensity, changing frequency (50 to 150 Hz) sound driven vibrating platform, 11 minutes 5 days/wk for 6 weeks. Blood samples were collected after euthanasia to determine creatinine, urea, calcium, phosphate (Pi), Alkaline Phosphatase, PTH (ELISA) and Scl (ELISA). Tibiae were resected and processed to obtain H&E stained sections and immunohistochemical (IHC) detection of Scl. BV/TV, Tb Th, Tb N, Tb. Sp, Cort Wi were determined in proximal metaphysis; osteocyte (Oc) density and Scl IHC in distal diaphysis for 15/21 specimens.Results: CKD & CKD+WBV specimens had creatinine, urea and Pi levels higher than controls, validating the CKD model; serum Scl was higher in CKD and a linear correlation with urea was found (R2=66.4%, p=