Location study of ermTR gene in Streptococcus agalactiae.

MARINA NOVOSAK; AGUSTIN MOYA; FERNANDO BOBADILLA; ILIANA JULIETA CORTESE; JAVIER LIOTTA; MARGARITA LACZESKI

Mar del Plata

Otro; Reunión conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE).; 2016

Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE).

Streptococcus agalactiae (GBS) is the leading cause of severe invasive infections in infants less than three months. Meningitis, pneumonia and sepsis are the leading cadres in these children. These infections are described as the most serious that an individual may suffer in their first hours of life, the maternal transmission is the main route of infection (95%).Although intrapartum antibiotic prophylaxis (IAP) with penicillin can significantly decrease neonatal GBS diseases, rates of resistance to antibiotics recommended for pregnant women allergic to penicillin, such as clindamycin and erythromycin, have increased. Among the mechanisms of resistance are changes in the target site mediated by erm genes frequently associated with transposons (conjugative and non-conjugative) on chromosome or plasmid.The genomic location of these genes is relevant for its potential interspecific horizontal transmission. The aim of this study was to determine the location of ermTR gene, one of the most reported in GBS. Three strains of GBS were selected for their history of phenotypic resistance and the presence of ermTR gene. The identification of GBS strains was confirmed by conventional biochemical tests. The extraction and purification of plasmid, cromosomal and total DNA were performed with commercial kits.The extraction control and/or chromosomal plasmids contamination was monitored by PCR with target in the 16S gene. Assays location of ermTR gene was performed using specific PCR. In the development of PCR for ermTR gene amplification a single sharp band of the expected size was obtained. Location assays show the presence of this ermTR gene into the chromosomal DNA of all the strains. In conclusion, the chromosomal location of the ermTR gene was determined and is necessary to continue with further studies of genomic context to contribute to the knowledge of resistance to macrolides and lincosamides.