Plasma cell-free DNA as a potential tool for obstetric outcomes prediction: quantification in pregnant and nonpregnant women

CEPEDA PJ; RACCA ME; MILESI MM; VARAYOUD J; CARDOZO MA; LUQUE EH; MUÑOZ-DE-TORO M; ROSSETTI MF; RAMOS J

Mar del Plata

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

SAIC

Cell-free DNA (cf-DNA) was associated with adverse obstetric outcomes prediction. We studiedcf-DNA levels variability in plasma (pl-) samples to evaluate its utility as a potential biomarker.Pl-samples from first (1st), second (2nd) and third (3rd) trimester pregnant (P, N=59) andnonpregnant (NP, N=24) women were used. Cf-DNA was obtained using QIAmp DNA blood minikit (QIAGEN). Actin-β (ACTB) gen was quantified by real-time quantitative PCR using an ACTBstandard curve. For PCR assays, 1000 ACTB standard copies were added to samples as an internalcontrol. Cf-DNA level was expressed as ACTB copy number and means±SD were statisticallycompared between groups. A correlation study between cf-DNA level and the gestational age(GA) at the delivery has been done. Cf-DNA increased in P respect to the NP group (2391±1388vs 1506±446 ACTB copies, respectively; p