Co-expressed genes profile in human metastatic melanoma cells associated with radioresistance and malignancy

ZUCCATO, CAMILA F.; NEGRIN, LARA M.; GARCÍA, FRANCISCO M.; BIOLATTI, VANESA; GRISSI, CECILIA; ATIA, MARIEL; MOLINARI, BEATRIZ; KLAMT, FABIO; CASTRO, MAURO; DURÁN, HEBE; IBAÑEZ, IRENE L.

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias, LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2017

Sociedad Argentina de Investigación Clínica

Melanomas are highly metastatic and though immunotherapy and targeted therapies have led to improvements in patient outcomes, the advanced disease has still a 5 year overall survival of less than 10%. Although melanoma is resistant, radiotherapy is usually used with systemic therapy for symptomatic metastases and as adjuvant therapy. In order to explore gene profiles associated with radioresistance and malignancy in melanoma, the aim of this study was evaluate gene sets involved in the intrinsic radiation response of melanoma cells with different degrees of malignancy but with the same genetic background (A375 and PCDNA3, non-invasive controls; G10, invasive and metastatic and A7, less aggressive). Bioinformatic analyses included expression microarrays data (Affymetrix) from these cells and a priori defined gene sets associated with DNA damage response (DDR) and invasion collected from KEGG and bibliography, to evaluate significant differences (FDR