Identification and characterization of differential gene expression in white blood cells exposed to ionizing radiation

BIOLATTI, VANESA; BELLORA, NICOLÁS; IBAÑEZ, IRENE L.

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias, LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2017

Sociedad Argentina de Investigación Clínica

The analysis of gene expression in blood cells has been emerged as a novel method for biodosimetry marker detection. The differential expressed genes could predict the clinical outcome of patients and guide the prevention, early diagnosis and treatment of ionizing radiation induced damage, improving traditional approaches. The aim of this study was to perform a meta-analysis of expression microarray data of irradiated blood cells in order to identify differentially expressed genes with overlapping among studies, which could be proposed as biomarkers of ionizing radiation exposure at 2 Gy and 24 h post-exposure. Data of four expression microarrays obtained from Gene Expression Omnibus (GEO) were evaluated by using the R programming language and Bioconductor packages. Differential gene expression was evaluated by Limma (parameters: lfc=1 and p