Robust and Scalable Multiplex Nanopore Sequencing

JOAQUIN EZPELETA; PILAR BULACIO; IGNACIO GARCIA LABARI; FLAVIA KRSTICEVIC; VICTORIA POSNER; GABRIELA VANINA VILLANOVA; ARRANZ SILVIA EDA; TAPIA ELISABETH

Conferencia; Nanopore Community Meeting.; 2019

Robust and Scalable Multiplex Nanopore Sequencing. Ezpeleta 1, P. Bulacio 1, I. Garcia Labari 1, F. Krsticevic 2,3, V. Emmel 4R. Hassan 4, V. Posner 5, V. Villanova 5,6, S. Arranz 5 and E. Tapia 1Robust and Scalable Multiplex Nanopore Sequencing1 Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas, CONICET-UNR, Argentina2 Entomology Department, The Hebrew University of Jerusalem, Israel3 Facultad Regional San Nicolás, Universidad Tecnológica Nacional, Argentina4 Laboratório de Oncovirologia, Instituto Nacional de Câncer, Río de Janeiro, Brasil5 Laboratorio Mixto de Biotecnología Acuática, CCTyE Acuario del Río Paraná, FCByF-UNR, Argentina6 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)TOWARDS MUX 4000 (36 nt), Crosstalk < 0.1%▪ 2 amplicons (A and B), each randomly tagged with 1959 barcodes▪ M=4096 hexa barcodes of 36 nt each (1959 x 2 + 178 negative controls)▪ 3918 DNA oligos of 79nt each obtained from LC Sciences (OligoMix®)▪ From attomoles (10-18) to nanomoles (10-9) - From 40K USD to 1K USD