(022) PREBIOTICS GALACTOOLIGOSACCHARIDES/ FRUCTOOLIGOSACCHARIDES (GOS/FOS®) MIXTURE, CALCIUM (Ca) ABSORPTIOM AND RESORPTION; AND NSUFFICIENCY OF Ca AND VITAMIN D (VD)

SEIJO, MARIANA; BONANNO MARINA SOLEDAD; BRYK, GABRIEL; ZENI CORONEL, ESTEFANÍA MAGALÍ; DE PORTELA, MARÍA LUZ PITA MARTÍN; ZENI, SUSANA NOEMÍ

CABA

Congreso; Reunión Anual de Sociedades de Biociencia; 2021

SAIC SAI AAFE NANOMED-AR

Prebiotics favors Ca absorption (CaAbs) and retention in bone. VD positively affects both processes. We evaluated if the prebiotic mixture assayed here exert similar effects under conditions of VD insufficiency and low Ca intake, using a preclinical model of postmenopausal bone loss. Adult ovariectomized rats fed a commercial diet during 15 days post-surgery. Then, for an additional 45-days period, 16 rats fed a VD-free (0 IU%) diet (-D groups) and 16 a normal VD diet (100 IU%) (+D groups). Both isocaloric diets content 0.3% of Ca (0.3%). At day-60, each group was subdivided into 2 groups which continuing feeding the same diet, having or not 0.25% of prebiotic mixture: +DPM and +D or -DPM and –D, respectively. We evaluated zoometric measurements, lactobacilli (LS) growth in feces by culture; activity of 4 fecal enzymes; cecal pH; CaAbs% by balance; femur Ca content (biochemically), bone volume fraction (BV/TV), epiphyseal cartilage total length (GPC.Th) and intestinal crypts depth (CD) by histology; total skeleton (TS) bone mineral content (TSBMC) and bone mineral density (TSBMD), lumbar spine (LS), proximal tibia (PrT) BMDs by densitometry. ANOVA and Bonferroni post hoc test were used to determine statistical significances. No differences in cecal pH, lactobacillus colonies, β-glucuronidase, urease and tryptophanase and β-glucosidase; CD; LS and PrTBMDs were observed between +DPM and -DPM; CaAbs, femur Ca content, TSBMC, TSBMD, TF BMD, BV/TV and GPC.Th were significantly higher in +DPM vs. -DPM (p