EFFECTS OF THE ADDITION OF TERIPARATIDE TO A BOVINE BONE GRAFTING MATERIAL ON THE REPAIR OF CRITICAL-SIZED BONE DEFECTS IN RATS. PRELIMINARY RESULTS

PELLEGRINI, G; BONANNO, MS; ZENI CORONEL E M; ORZUZA, R.; AVEDAÑO, ME; ZENI SN.

MAR DEL PLATA

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2022; 2022

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS)

We previously determined the osteoconductive effect of a bovinebone graft manufactured in Argentina [Synergy Bone Matrix, OdontitImplant Systems, Argentina (SBM)], on the bone healing processin an experimental model in rats. Several studies have shown thatintermittently administered PTH has an anabolic effect on cancellous and cortical bone. On these bases, we hypothesized that theaddition of the synthetic PTH analog, Teriparatide (TPT), to SBMcould accelerate bone regeneration. The aim of the present experimental study was to evaluate if the addition of TPT to SBM presentsany additional beneficial effect versus the use of SBM alone. Thirtyadult male Wistar rats of 333±39.22 (n=6/group) and a critical-sizebone defect (CSD) was created in the medial aspect of both tibiae.All CSDs received one of the following treatments for 30 days: G1:control without treatment; G2: SBM (Lot No: E11121216); G3: SBMmixed with 20 µg of T (Osteofortil, BioSidus, Argentina); G4: injection of 0.125 µg/day of TPT and G5: SBM mixed with 20 µg of TPTand SC injection of 0.125 µg/day of the same drug. The animalswere sacrificed 45 days after the beginning of the experiment. Weanalyzed serum PTH, P1NP and CTX (ELISA). At the end of thestudy, the tibiae were evaluated with X-ray microtomography (µCT).Serum PTH levels (pg/mL) at the end of the study were 4.6±1.2a;11.3±2.3b; 16.4±0.7c; 17.9±0.8c and 19.1±0.5c, respectively, P1NPlevels (pg/L) at the end of the study were 2.24±0.24a; 2.63±0.16c;2.54±0.37c; 2.43±0.41b; 2.48±0.45b; respectively. Preliminary µCTresults showed: G1 no bone defect repair; G2: poor repair; G3: repair; G4: overgrowth of bone tissue around the bone callus and G5:repair without bone overgrowth. Our findings showed that the addition of TPT to SBM mixture accelerated the bone repair processwhile the daily injection did not produce any additional improvementin the bone regeneration exerted by SBM.