INVESTIGADORES
DI CONZA Jose Alejandro
congresos y reuniones científicas
Título:
Characterization of an Escherichia coli strain co-expressing different β-lactamases, FosA3 and MCR-1 resistance mechanisms from healthy chicken
Autor/es:
GONZALES ESCALANTE, EDGAR; GUTKIND GABRIEL; DI CONZA JOSÉ
Lugar:
Washington
Reunión:
Congreso; ASM Microbe 2022; 2022
Institución organizadora:
ASM
Resumen:
Antimicrobial drugs used in food animals exceed, by far, human use, including therapeutic, prophylactic and metaphylactic purposes. Even if banned in several countries, they have been extensively used as growth promoters. The emergence of multidrug-resistant (MDR) Enterobacterales has been reported in farm animals, comprising some of the most clinically relevant antibiotics. The transmission of these resistance genes that can be captured by the human microbiota is a worrisome scenario.A collection of 200 cefotaxime-resistant strains were recovered from cloacal swabs of healthy chickens after selection on Mac Conkey Agar supplemented with cefotaxime (4 µg/ml) in November 2015, from a chicken farm in Peru. Susceptibility was analyzed by disk diffusion or microdilution accordingly to CLSI. Phylogenetic group was obtained by PCR, and genes encoding different resistance markers were characterized by PCR and sequencing. E. coli P79 exhibited a MDR profile (resistance to ceftazidime, cefotaxime, cefoxitin, amoxicillin/acid clavulanic, chloramphenicol, gentamicin, fosfomycin, colistin, and trimethoprim-sulfamethoxazole) but remained susceptible to amikacin, ciprofloxacin, imipenem, and meropenem. This isolate belonged to phylogenetic group D, and the presence of resistance markers to β-lactams (blaCTX-M-2, blaCMY-2 and blaDHA-1), fosfomicyn (fosA3) and colistin (mcr-1.1) was detected.All these resistance genes can be located on transferable plasmids facilitating their spread to primary human pathogens.In summary, for a better comprehension of the spread of clinically relevant MDR clones, and in accordance with the universally shared One Health approach, it seems essential to improve epidemiological surveillance in veterinary medicine, in farm animals and in the environmental setting.
