Neurosphere approach to understand CNS regeneration

MOLINARI YAMILA A.; PIBUEL MATIAS; BYRNE AGUSTIN J; POODTS DANIELA; DIAZ MARIANGELES; LOMPARDIA SILVINA; SILVESTROFF LUCAS; FRANCO PAULA G.

Encuentro; XXXV Annual Meeting of the Argentinian Society for Neuroscience; 2020

Neural stem and progenitor cells (NSC/NPC) from the subventricular zone (SVZ) are able to generate all neural cell types and can be cultured as freefloatingaggregates called neurospheres (NS).NS cultures are useful tools to model the cellular processes that take place during endogenous repairment that occur in the central nervous system (CNS) in a sequential and orderly manner. In thepresent work, we first developed NS cultures to evaluate the effect of the demyelinating agent Cuprizone (CPZ) on NSC/NPC behavior.CPZ treatment of NS cultures revealed its direct effect on NSC/NPCs proliferation and differentiation mechanisms. The finding that cell migration was also affected by CPZ, led us to investigateextracellular matrix (ECM) involvement in the control of cell migration from NS.