Una Enfermedad de Transmisión Alimentaria por Salmonella Enteritidis durante la gestación tardía induce restricción del crecimiento fetal intrauterino

BETANCOURT D; HERNÁNDEZ BRIDÓN, NAIROBI; GAROFALO AILIN; SARNACKI SH; CERQUETTI MC; PUSTOVRH MC,; GIACOMODONATO, MN; NOTO LLANA M

Cali

Congreso; XXIV Simposio de Investigaciones en Salud; 2022

Facultad de Salud, Universidad del Valle

Introduction: Salmonella foodborne disease during pregnancy causes a significant fetalloss in domestic livestock and preterm birth, chorioamnionitis and miscarriage inhumans. These complications could be is associated with alterations in placentalstructure. This study was aimed to determine whether a low dose of SalmonellaEnteritidis during late gestation affects placental histomorphometric in mice.Methods: We used a self-limiting enterocolitis murine model. BALB/c pregnantanimals received a very low dose of Salmonella Enteritidis (3-4 x 10 2 CFU/mouse) ongestational day (GD) 15. At day 3 post infection bacterial loads, serum cytokinesexpression and some placental histomorphometrics parameters were analysed.Results: We found that a sub-lethal infection with Salmonella induced a considerableinfection of maternal organs leading to placental morphometric alterations, includingincreased placental thickness, reduced surface area, and diminished major and minordiameters. In response to infection we observed a significant reduction in fetal weight -to-placental weight-ratio and an increase in the placental coefficient. Also, theexpression of IFN-gamma and IL-6 was up regulated in response to Salmonella inmaternal serum. Furthermore, foci of necrosis accompanied by acute leukocyteinfiltration in decidual zone, reduction of vascular spaces and vascular congestion inlabyrinth zone, were also evident in placentas from infected mothers on GD 18. Ourdata shows that placentas from infected mothers are phenotypically different fromcontrol ones.Discussion: Our findings demonstrated that low doses of Salmonella during lategestation alters the placental morphometry leading to negative consequences onpregnancy outcome such us significant reduced fetal body weight.