Effect of a hypercaloric diet on hypoandrogynous rat lung

BIAGGIO VERONICA; ABALLAY, FEDERICO; SALINAS MARYSOL; ZELARAYAN SARMIENTO, DANIELA; PIGUILLEM, SILVANA; CIMINARI, MARIA EUGENIA; RAZZETO, GABRIELA; SALINAS, ELOY; ALVAREZ, SILVINA; PEREZ CHACA, MARÍA VERONICA; GOMEZ, NIDIA NOEMI

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2022

iedad Argentina de Investigación Clínica

Obesity is a systemic state of inflammation and oxidative stress that affects normal respiratory functioning. The presence of androgen receptors in lung reveal that these hormones might play a key role in lung physiology. Obesity-mediated oxidative stress produced in adipose tissue is one of the main factors considered as oxidant source and inflammation mediator. The aim of this work was to study the effect of diet-induced obesity on the lung of androgen-deficient rats (castrated). Wistar male rats (200 ± 20 g) were separated in four groups: Control with normal diet (CoN), castrated with normal diet (KN), control with hypercaloric diet (CoOB) and castrated with hypercaloric diet (KOB) and sacrificed 30 days after castration. Biochemical parameters were analysed in serum and the expression of antioxidant enzymes and NOX-2, FOXO, HO-1 and RA in lung. ANOVA and Tukey test were used for statistical analyses. The results showed TBARS levels increased in KOB group compared to CoN (p<0.001) and KN (p<0, 01) groups, respectively. CAT activity was increased in KN (p<0.05) group. HDL levels increased in CoOB (p<0.001), KN (p<0.001) and KOB (p<0.01) groups. Both, urea and TG determinations were increased in KN (p<0.001); p<0.01 and KOB (p<0.001) groups. CL was increased in CoOB (p<0.001); KOB (p<0.01) compared to CoN group and increased in KN (p<0.001) and KOB (p<0.001) compared to CoOB group. CAT expression decreased in CoOB and KN groups, and RA expression increased in KN group compared to control group. Antioxidant enzymes NOX-2 and SOD-2 (p<0.01) and GPx-1(p<0,05) increased in KOB group compared to control. FOXO-1 and HO-1increased in castrated group, with obesogenic diet (p<0.01). We previously demonstrated an important oxidative stress state in a castrated animal model. In conclusion, obesity added to androgen deficiency modifies different serum parameters. In fact, some inflammatory molecular pathways reveal a potential relationship between both situations (androgen deficiency-obesity).