Estimation of acute oral toxicity of pesticides products in 3t3 cell line

MARIELA LENZE; JUAN IGNACION PINA; MARTINA BENEDETTI; AGUSTINA RESCHINI; MARTÍN NICOLÁS RIVERO; SILVIA WIKINSKI; MARÍA LAURA GUTIÉRREZ

Otro; Summer School on Innovative Approaches in Science; 2022

Physicians Committee for Responsible Medicine

SENASA (National Service of Food Health and Quality) is the national organism in Argentina responsible for executing policies on animal and plant health and quality and food safety. One of the endpoints demanded for the registration of active ingredients and formulated products is rodent lethal dose acute oral toxicity (LD50 value) and the classification system proposed by the World Health Organization (WHO) is used. In this study we compared the LD50 estimated in vitro with the in vivo one obtained in rodents to evaluate if the in vitro test is suitable to estimate the initial dose for an in vivo acute oral toxicity test for pesticides products to significantly reduce the number of animals.Four pesticides and the in vivo data obtained by the OECD Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure were provided by a local company (ATANOR S.C.A). The in vitro data was obtained through the implementation of the “Guidance document on using cytotoxicity test to estimate starting doses for acute systemic toxicity tests” (TG 129, OECD).IC50 values (µg/mL) obtained four each product (named by a code number) was: 1331(#4); 187.85(#10); 11.61(#11) and 5096.5(#13). Next, we estimated the LD50 values (mg/Kg): 1535.3(#4); 740.43(#10); 263.1(#11) and 2529.91(#13); which would correspond to class II, II, II and III respectively, according to the WHO classification. On the other hand, the classification obtained by the in vivo data was III, II, III and IV respectively.These preliminary results indicate that the in vitro method could be implemented to predict LD50 values of pesticides complex formulations for use as the starting dose in the in vivo procedure. Finally, we expect to expand the number of pesticides products and add household products to improve the prediction of the in vitro acute oral toxicity assessment.