PORPHYRINOGENIC AGENTS AFFECT MITOCHONDRIAL FUNCTION IN A MOUSE MODEL OF ACUTE INTERMITTENT PORPHYRIA

ZUCCOLI, JOHANNA; MARTINEZ MARIA DEL CARMEN; RUSPINI, SILVINA; BATLLE ALCIRA; BUZALEH ANA MARIA

Mar del Plata

Congreso; Reunion Conjunta de Biociencias; 2019

Sociedad Argentina de Investigacion Clinica

The mitochondria playS a vital role in energy metabolism.In the inner mitochondrial membrane, the redox reactions of oxidative phosphorylation and the ATP synthesis are performed. Heme deficiency would trigger severe cell damage. Moreover, heme is a prosthetic group of a numerous hemeproteins such as Cytochrome c oxidase (Cox, Complex IV), succinate dehydrogenase (complex II), ubiquinol-cytochrome c reductase (complex III) and mitochondrial nitric oxide synthase isoform (mtNOS). The aim was to further investigate the effects of volatile anaesthetic and others porphyrinogenic agents on mitochondrial metabolism in a mouse model of AIP. For this purpose we determined the level of expression of Complex IV, Complex V and nitric oxide metabolism. Also, the mitochondrial integrity markers were evaluated. Experiments were performed in brain mitochondria of AIP genetic male mice model receiving Isoflurane (2 ml/kg), Sevoflurane (1,5ml/kg), ethanol (30%), allylisopropylacetamide (AIA, 350 mg/kg), Veronal (167 mg/kg) or starved (24 hours). termined. Complex IV expression was only diminished by Veronal (50%, p