599. (590) MODULATION OF EXTRACELLULAR PH IN CYSTIC FIBROSIS CELLS BY EGFR

MASSIP-COPIZ, MARÍA MACARENA; VALDIVIESO, ANGEL G.; ASENSIO CRISTIAN JORGE ALEJANDRO; SANTA-COLOMA, TOMÁS A.

virtual

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

SAIC, SAI, AAFE, NANOMED-AR

Cystic fibrosis (CF) is an inherited disease caused by mutations in the CFTR (CF transmembrane conductance regulator) gene. It is principally characterized by chronic infections and persistent inflammation in lungs. One of the hypotheses that tried to explain the increased susceptibility to lung infections is the reduction in the airway surface liquid (ASL) pH. The changes observed in the extracellular pH (pHe) could be a consequence of a reduced bicarbonate transport through CFTR and also an increase in lactic acid secretion. Considering that the EGFR pathway is affected in the CF phenotype, the aim of the present work was to determine if the EGFR pathway is involved in the extracellular pH regulation in CF cells. Two cellular models were used: IB3-1 cells (CF cells) and C38 cells (IB3-1 “corrected” cells). Together with the extracellular pH and lactic acid (lactate) secretion measurements, LDH expression and activity were performed by RT-PCR, Western Blot, flow cytometry, confocal microscopy and spectrophotometric techniques. The results reported a decrease in pH in the extracellular medium culture (p