Proteomic study of breast cancer cell line after HO-1 modulation by hemin treatment

COLÓ, G.P.; FÄSSLER R.; SCHWEITZER, K.; ALONSO, E.G.; CURINO, A.C.; FERNÁNDEZ CHÁVEZ, L.; FACCHINETTI, M.M.

virtual

Congreso; Reunión Anual de Sociedades de Biociencia; 2021

Sociedad Argentina de Investigación Clinica

Hemoxygenase-1 (HO-1) is a microsomal enzyme that catalyzes the degradation of the heme group and it can be translocated to multiple subcellular compartments. Our laboratory, among others, has shown that HO-1 regulates several processes related to cancer progression such as: proliferation, invasion migration, metastasis and the epithelial-mesenchymal transition. The aim of this work is to investigate the role of HO-1 in the proteome modulation in a breast cancer cell line. Protein extracts of LM3 cell line treated with hemin, a pharmacological HO-1 inducer (80µM, 24h), were obtained and studied by Western blot and Mass Spectrometry (MS). MS-data analysis showed 1033 from 7292 proteins were modulated after hemin treatment (ANOVA p