Protective effect of naringin in an experimental model of hypogonadism associated with type 1 Diabetes mellitus

MENA C; RODRÍGUEZ V; MOINE L; SILVANO L; TOLOSA DE TALAMONI N; DIAZ DE BARBOZA G

Córdoba

Jornada; XXIII Jornada de Investigación Científica; 2022

FCM-UNC

Hypogonadism is a complication associated with type 1 Diabetes mellitus (DM1). Hyperglycemia and lack of insulin trigger oxidative and nitrosative stress weakening the Leydig cells, which produce testosterone. Our objective was to evaluate the effect of the flavonoid naringin (NAR) as a protector of Leydig cells in an experimental model of DM1. Control male Wistar rats (C), rats treated with 60 mg streptozotocin/kg b.w. (STZ) and STZ rats treated with NAR (20, 40 or 80 mg/kg b.w., STZ+NAR) were employed. After 30 days of treatment, the rats were sacrificed and blood and testes were obtained. Blood glucose, HbA1c and serum testosterone were determined. Glutathione content (GSH) and catalase (CAT) activity were quantified in testis homogenates. In testicular sections, the expression of the inducible enzyme nitric oxide synthase (iNOS) was evaluated by immunohistochemistry and cell apoptosis by TUNEL. The results were analyzed by ANOVA/Tukey (p