POSITIVE KIT BOVINE CELLS IN MUSCULAR TUNIC OF THE ABOMASUM AT THREE DIFFERENT AGES

MÁRQUEZ SG, GALOTTA JM, GÁLVEZ GA, PORTIANSKY EL, BARBEITO CG

Mendoza

Encuentro; XXVIII Annual Scientific Meeting Sociedad de Biología de Cuyo; 2010

Sociedad de Biología de Cuyo

Interstitial cells of Cajal (ICCs) are widely studied, but there is a scarce amount of information about these cells at different ages. In the present study, morphometric parameters of ICCs on the pyloric region of abomasum has been established and compared at three stages of organ development: fetus (20¡¾2 weeks), one week calf and adult bovine. Anti c-Kit immunochemical technique was realized to identify ICC specifically. Analysis of Variance (ANOVA) and Tukey¡¯s test were applied to determine signification (P<0,05). Positive c-Kit cell bodies measure 49,31¡¾11,48 ¥ìm¢©, major axis 14,54¡¾2,21 ¥ìm, minor axis 4,37¡¾0,64 ¥ìm, perimeter 33,69¡¾4,66¡¾2 weeks), one week calf and adult bovine. Anti c-Kit immunochemical technique was realized to identify ICC specifically. Analysis of Variance (ANOVA) and Tukey¡¯s test were applied to determine signification (P<0,05). Positive c-Kit cell bodies measure 49,31¡¾11,48 ¥ìm¢©, major axis 14,54¡¾2,21 ¥ìm, minor axis 4,37¡¾0,64 ¥ìm, perimeter 33,69¡¾4,66¡¾11,48 ¥ìm¢©, major axis 14,54¡¾2,21 ¥ìm, minor axis 4,37¡¾0,64 ¥ìm, perimeter 33,69¡¾4,66¡¾2,21 ¥ìm, minor axis 4,37¡¾0,64 ¥ìm, perimeter 33,69¡¾4,66 ¥ìm in fetus; area 61,61 ¡¾ 10,12 ¥ìm¢©, major axis 14,92 ¡¾ 1,77 ¥ìm, minor axis 5,37 ¡¾ 0,29 ¥ìm, perimeter 35,53 ¡¾ 3,90 ¥ìm for calf and area 30,95 ¡¾ 4,55 ¥ìm¢©, major axis 10,55 ¡¾ 1,17 ¥ìm, minor axis 3,87 ¡¾ 0,34 ¥ìm, perimeter 24,76 ¡¾ 2,25 ¥ìm in adult animal. The statistical comparison shows all the parameters get smaller from calf to adult. On the other hand, area and minor axis get bigger from fetus to calf, whereas their major axis and perimeters are similar. These differences could be related to the differential growing of abomasums during suckling stage, whereas smaller dimensions of the adult could be connected with a different relation between cell body and its prolongations.m in fetus; area 61,61 ¡¾ 10,12 ¥ìm¢©, major axis 14,92 ¡¾ 1,77 ¥ìm, minor axis 5,37 ¡¾ 0,29 ¥ìm, perimeter 35,53 ¡¾ 3,90 ¥ìm for calf and area 30,95 ¡¾ 4,55 ¥ìm¢©, major axis 10,55 ¡¾ 1,17 ¥ìm, minor axis 3,87 ¡¾ 0,34 ¥ìm, perimeter 24,76 ¡¾ 2,25 ¥ìm in adult animal. The statistical comparison shows all the parameters get smaller from calf to adult. On the other hand, area and minor axis get bigger from fetus to calf, whereas their major axis and perimeters are similar. These differences could be related to the differential growing of abomasums during suckling stage, whereas smaller dimensions of the adult could be connected with a different relation between cell body and its prolongations.¡¾ 0,29 ¥ìm, perimeter 35,53 ¡¾ 3,90 ¥ìm for calf and area 30,95 ¡¾ 4,55 ¥ìm¢©, major axis 10,55 ¡¾ 1,17 ¥ìm, minor axis 3,87 ¡¾ 0,34 ¥ìm, perimeter 24,76 ¡¾ 2,25 ¥ìm in adult animal. The statistical comparison shows all the parameters get smaller from calf to adult. On the other hand, area and minor axis get bigger from fetus to calf, whereas their major axis and perimeters are similar. These differences could be related to the differential growing of abomasums during suckling stage, whereas smaller dimensions of the adult could be connected with a different relation between cell body and its prolongations.¡¾ 4,55 ¥ìm¢©, major axis 10,55 ¡¾ 1,17 ¥ìm, minor axis 3,87 ¡¾ 0,34 ¥ìm, perimeter 24,76 ¡¾ 2,25 ¥ìm in adult animal. The statistical comparison shows all the parameters get smaller from calf to adult. On the other hand, area and minor axis get bigger from fetus to calf, whereas their major axis and perimeters are similar. These differences could be related to the differential growing of abomasums during suckling stage, whereas smaller dimensions of the adult could be connected with a different relation between cell body and its prolongations.¡¾ 0,34 ¥ìm, perimeter 24,76 ¡¾ 2,25 ¥ìm in adult animal. The statistical comparison shows all the parameters get smaller from calf to adult. On the other hand, area and minor axis get bigger from fetus to calf, whereas their major axis and perimeters are similar. These differences could be related to the differential growing of abomasums during suckling stage, whereas smaller dimensions of the adult could be connected with a different relation between cell body and its prolongations.