INVESTIGADORES
BARBEITO Claudio Gustavo
congresos y reuniones científicas
Título:
PLACENTAL LESIONS AND EMBRYONIC DEATH GENERATED BY ACUTE CADMIUM INTOXICATION IN RATS AT 15 DAYS OF PREGNANCY
Autor/es:
DÍAZ M, ZANUZZI C, GONZÁLEZ N, GÓMEZ S, BOSCHETTI L, QUIROGA M, GIMENO E, NAJLE R, BARBEITO C
Lugar:
Buenos Aires
Reunión:
Jornada; Décima segunda Jornada Multidisciplinaria de la Sociedad Argentina de Biología; 2010
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
Cadmium accumulation in the placenta alters its structure and affects
prenatal development. In order to analyze uterine, placental
and embryonic parameters Wistar rats were injected with 10 mg
Cd+2/kg of body weight on 4 (G4), 7 (G7) or 10 (G10) days of
pregnancy and sacrificed at 15 days of gestation. The weight of the
embryonic vesicles, embryos and placentas was recorded. Macroscopic
observations were performed to determine resorptions and
uterine and fetal alterations. Some samples of placentas were processed
for lectinhistochemestry. Only the weight of the placentas
from G7 and G10 females was lower compared with controls. All
intoxicated groups showed significantly higher number of
resorptions than controls. Placentas from Cd-treated dams showed
hemorrhage, congestion and necrosis. The carbohydrate pattern
showed positive labeling for PNA, UEAI, and SBA lectins only in
the intoxicated females. We confirmed the deleterious cadmium
effect on placental structure and prenatal ontogeny of the rat and
further demonstrated the changes in the pattern of placental carbohydrates
at 15 days of pregnancy
pregnancy and sacrificed at 15 days of gestation. The weight of the
embryonic vesicles, embryos and placentas was recorded. Macroscopic
observations were performed to determine resorptions and
uterine and fetal alterations. Some samples of placentas were processed
for lectinhistochemestry. Only the weight of the placentas
from G7 and G10 females was lower compared with controls. All
intoxicated groups showed significantly higher number of
resorptions than controls. Placentas from Cd-treated dams showed
hemorrhage, congestion and necrosis. The carbohydrate pattern
showed positive labeling for PNA, UEAI, and SBA lectins only in
the intoxicated females. We confirmed the deleterious cadmium
effect on placental structure and prenatal ontogeny of the rat and
further demonstrated the changes in the pattern of placental carbohydrates
at 15 days of pregnancy
pregnancy and sacrificed at 15 days of gestation. The weight of the
embryonic vesicles, embryos and placentas was recorded. Macroscopic
observations were performed to determine resorptions and
uterine and fetal alterations. Some samples of placentas were processed
for lectinhistochemestry. Only the weight of the placentas
from G7 and G10 females was lower compared with controls. All
intoxicated groups showed significantly higher number of
resorptions than controls. Placentas from Cd-treated dams showed
hemorrhage, congestion and necrosis. The carbohydrate pattern
showed positive labeling for PNA, UEAI, and SBA lectins only in
the intoxicated females. We confirmed the deleterious cadmium
effect on placental structure and prenatal ontogeny of the rat and
further demonstrated the changes in the pattern of placental carbohydrates
at 15 days of pregnancy
+2/kg of body weight on 4 (G4), 7 (G7) or 10 (G10) days of
pregnancy and sacrificed at 15 days of gestation. The weight of the
embryonic vesicles, embryos and placentas was recorded. Macroscopic
observations were performed to determine resorptions and
uterine and fetal alterations. Some samples of placentas were processed
for lectinhistochemestry. Only the weight of the placentas
from G7 and G10 females was lower compared with controls. All
intoxicated groups showed significantly higher number of
resorptions than controls. Placentas from Cd-treated dams showed
hemorrhage, congestion and necrosis. The carbohydrate pattern
showed positive labeling for PNA, UEAI, and SBA lectins only in
the intoxicated females. We confirmed the deleterious cadmium
effect on placental structure and prenatal ontogeny of the rat and
further demonstrated the changes in the pattern of placental carbohydrates
at 15 days of pregnancy
