In vitro cytotoxic preliminar studies of aqueous extracts of Achyrocline satureioides on vero cells

SABINI C.; ESCOBAR F.; CONTIGIANI M.; ZANON S.; SABINI L.

Tafi del Valle, Tucumán

Jornada; XXV Jornadas Científicas Organizadas Por La Asociación De Biología De Tucumán; 2008

Introduction: The Herpesviridae family produces latent infections that could be reactivated after long periods of time. Antiviral agents such as Aciclovir generate resistance in the virus so that the search for new therapeutical alternatives to be used alone or in combination is necessary for the treatment of this pathology. In this way, medicinal plants constitute a promising source of active compunds. Several bioactive properties -antiinflamatory, sedative, hepatoprotective, antioxidant, inmunomodulating, antitumoral and antimicrobialhave been attributed to A. satureioides. However, the study of the cytotoxic potential of this plant has not been documented yet. Objetive: To evaluate in vitro the cytotoxic action on Vero cells of aqueous extracts of A.satureioides. Materials and Methods: Aqueous extracts were obtained at 4°C (CAE) and 70°C (HAE). The MCNC was studied at different concentrations of the extracts and CC50 was determined by the Neutral Red uptake assay (NR) and the MTT salt reduction assay. Statistics/linear regression analysis was performed for a determination coeficient >0.90 (Origin 6.0). Results: The MCNC and CC50 by the RN and MTT for CAE were: 480 ug/ml, 960 ug/ml and >1900 ug/ml and for HAE 260 ug/ml, 373 ug/ml and 559 ug/ml, respectively. Discussion and conclusions: The NR and MTT methods were more sensitive than the MCNC one. They showed that both extracts could alter the phagocytary ability of the cell. These results will help to define the selectivity action of the extracts for antimicrobial treatments.