Study of cytotoxic parameters of Verbascum thapsus extracts on vero cells

ESCOBAR F.; GALLOTTI V.; SABINI C.; TORRES C.; TONN C.; GIORDANO O.; SABINI L.

Tafi del Valle, Tucumán

Jornada; XXV Jornadas Científicas Organizadas Por La Asociación De Biología De Tucumán; 2008

Introduction: Verbascum thapsus L. Scrophulariacea (verbascum) is a medicinal plant native of Europe and Asia. It has been described as having expectorant, astringent, febrifugue, descongestive and antialergic properties. However, the use of this plant in vivo needs the evaluation of cytotoxic parameters to guarantee its safety. Aims: To study the cytotoxicity of 5 vegetable extracts (VE) from V.thapsus. Materials and Methods: n-hexanic (HE), cloroformic (CE), methanolic (ME), cold aqueous (CAE) and hot aqueous extracts (HAE) were obtained from leaves. They were diluted in MEM-Eagle-Earle + 2% BFS and seeded on Vero cells monolayers. MCNC and CC50 by neutro red and MTT were calculated. Results: A correlation was observed between the 3 methods employed, so a toxicity order of the VE could be defined: EAF