Comparative assays of cytotoxicity induced by extracts of Verbascum thapsus

ESCOBAR F.; GALLOTTI V.; SALAS M.; SABINI C.; GIORDANO O.; CONTIGIANI M.; SABINI L.

Huerta Grande. Córdoba

Encuentro; Primera Reunión Conjunta de Sociedades de Biología de la República Argentina; 2007

Introduction: The biotechnological application of V. thapsus in the phytomedicinal field is promissory to control the microbial infections, which demands to study its selectivity of action. Objective: To analyze the cytotoxic effect of 5 vegetal extracts (VE) obtained from Verbascum thapsus (Ambay). The extracts: n-hexanic (HE), chloroformic (CE), methanolic (ME), cold and warms aqueous extract (CAE and WAE), were obtained from leaves of the plant, by continuous and sequential agitation. The toxicity assays were carried out in plates of 96 wells; 0.2 mL/well of different concentrations of each VE [10-4000 μg/ml] were seeded on monolayer Vero cells. Controls wells without VE were included. The system was incubated to 37°C by 48 hs. The Maximum non Cytotoxic Concentration (MCNC) was determinate by daily examination at the optical microscopic. The CC50 was calculated by neutral red uptake method (NRU). Statistical analysis of lineal regression was made, for a coefficient of determination 0.90 (software Origin 7,5). Although by both techniques the toxic concentrations surpassed 1mg/ml, NRU showed to be more sensitive to evaluate toxicity on eukaryotic cells. A toxicity order was established: WAE