Antiretrovirals on THP-1 inflammation markers

GROSSO T; DE MARZI M; GUERRA LN; SUED O

Congreso; The future of Redox Biology Congress; 2022

Society for Free Radical Research Europe

Antiretroviral drugs are widely known that produce oxidative stress on patients treated for HIV infection. We evaluated the effects of Darunavir (DRV, 0,055mM), Tenofovir Alafenamide (TAF, 7,14mM) and Dolutegravir (DTG, 0,072mM) on THP-1 cells. Cell viability, interleukin-8 (IL-8) production and possible activation of these monocytes by nitrites secretion (N) were measured. After 48h treatment, we observed no effect with DRV. On the other hand, DTG increased both markers compared to vehicle control (CTRL) (IL-8: 2998 ±748,0 pg/ml vs 1201±119 pg/ml [CTRL], p