INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cyclic AMP, calmodulin and calcium implication in Xenopus laevis vitellogenin uptake
Lugar:
Puerto Madryn
Reunión:
Congreso; SAIB; 2010
Resumen:
The transformation of oogonia into oocytes is commonly
described as oogenesis. This is the differentiation of a precisely organized,
fully mature and functioning oocyte, which is a result of a complex series of
specific cellular and molecular events.
Vitellogenesis is one of the most important processes
during the oogenesis phase in oviparous vertebrates. It is characterized by
hepatic production of the glycoprotein vitellogenin, which is transported via
the bloodstream to the ovary where the oocytes enter through receptor-mediated
endocytosis.
In previous works we established that in the amphibian
Xenopus laevis, cAMP is the diffusible
signal molecule that is capable of passing through fully open heterologous gap
junctions that trigger the vitellogenic process. In addition, we demonstrated a
participation of the calcium binding protein Calmodulin (CaM) during X. laevis vitellogenin uptake.
In order to assess the physiological roles of cAMP,
CaM and Calcium during X. laevis
vitellogenesis, experiments with chemical antagonists/agonists were performed.
We showed that the cAMP molecule is upstream in the signaling
pathway with regard to the Calmodulin/Calcium complex.