In vitro anti-helicobacter pylori action of decoction of Minthostachys verticillata

ESCOBAR F.; VOGT M.V.; MATTANA C.; VEGA M.; DEMO M.; SABINI L.

Villa Giardino, Córdoba

Jornada; XV Jornadas de Biología; 2005

The human bacterial pathogen Helicobacter pylori (HP) has been recognized as a major causative factor in peptic ulcer diseased. It is known that HP could be eradicated by a combination of therapeu-tic agents such as antibiotics, bismuth subsalicylate, protom pump inhibitors and H2-blockers. However, the cure is incomplete and undesirable side effects are certain to occur. On the other hand, the anti-HP activity of the Minthostachys verticillata (peperina), a traditional herbal medicine, has not been studied. The vegetable decoction was obtained according to Mongelli et al (1995), its inhibitory action was evaluated in vitro against two strains of HP tox+ producer vacA toxin, both isolated of severe gastritis cases. The screening test was made by the technique of radial strikes, and the CIM determination was made by the disk diffusion method. Both strains were sensible to the inhibitory action of M verticillata decoction, with 9.19 mg/ml value CIM and 15 mm ratio inhibition halos. Greater concentrations show inhibition halos of 30 mm or higher, similar to the positive control Amoxiciline. The relation of the cytotoxic concentration of the decoction on eukaryotic cells evaluated on previous assays (Escobar et al, 2004) and the active concentration against HP, threw a therapeutic Index >1. Data reveals the potential phitomedicinal application of M. verticillata decoction to control infection of Helicobacter pylori.