ESTROGENS MODULATE THE EXPRESSION OF LYSOSOMAL PROTEINS IN A RAT MODEL OF PARKINSON´S DISEASE.

LEIVA N; BONNACORSO MP; MEIER G; CARVELLI L,; SOSA MA; CABRERA R

Congreso; XXXIX Reunión Anual de la Sociedad de Biología de Cuyo; 2021

Parkinson's disease (PD) is a neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons fromSubstance Nigra Compacta. A genetic study identified 24 locis that are associated with PD, 11 of the 24 genes are involvedor disrupt various functions of the autophagic-lysosomal pathways. Lysosomes participate in the degradation ofmacromolecules from endocytosis and autophagy processes. Epidemiological and clinical studies reveal a difference in thedevelopment of PD between genders, giving sex hormones a neuroprotective function and making them an interestingtherapeutic proposal. The objective of our work was analyze the effect of estrogens on the expression of lysosomal proteinsin a rat model with the phenotype of PD. Two month old male Sprague-Dawley rats underwent stereotaxic surgery toadminister 6-hydroxydopamine (6-OHDA) or artificial cerebrospinal fluid (V) to the left striatum. After 7 days, they receivedchronic treatment for 10 days with 17-β-Estradiol (E) or V. The groups were made up of: C (V lesion); E (V + E lesion); HP(6-OHDA lesion) and HPE (6-OHDA + E lesion). After the treatments, the animals were sacrificed and the left and rightbrain regions: substantia nigra and prefrontal cortex were extracted and homogenized. The samples were processed forimmunoblotting using antibodies anti- cathepsin D (CatD) and anti-actin antibodies. Preliminary results show that chronicestrogen treatment increases CatD and actin expression both in substantia nigra and prefrontal cortex. Since the CatD proteasereduces the concentration of the α-synuclein protein in PD, the present results suggest that the increase in lysosomal functionwould exert neuroprotective effects in cells affected by the disease. Likewise, it should be mentioned that estrogens couldalso modulate the organization of the cytoskeleton, as a stage of neuromodulation