THE NEUROTOXIN OF AN AUTOCHTHONOUS CLOSTRIDIUM BOTULINUM AFFECTS THE ACTIN CYTOSKELETON IN BREAST CANCER CELLS

GUARNIOLO D; PATRICIA CABALLERO; CHAPANA A; SOSA ESTEBAN; RA FERNANDEZ; MIGUEL A. SOSA; CARVELLI L,

Congreso; XXXVII Reunión Anual de la Sociedad de Biología de Cuyo; 2019

Sociedad de Biología de Cuyo

(Botox®), so in the future, they could be used as a therapeutic agent. The NTBos mechanism of action on certain pathologies is stillto be clarified. Previous results from our laboratory showed that autochthonous NTBo 1935 from Su, degrades actin of rat brainhomogenates, suggesting this protein could be an active target of NTBos. In this work, the action of this NTBo on the actincytoskeleton in mammary tumor cells was evaluated. The NTBos of Su from strain 1935 and strain A Hall (both serotype A) werepurified by saline precipitation. MCF7 cells (breast cancer cells) were cultured in Petri dishes or coverslips with 250 LD50 of theNTBos for 25, 45, or 90 min. After incubations, cells were processed for Western blot or immunofluorescence in order to evaluatethe distribution and expression of actin. NTBo 1935 produced higher actin degradation and an increased location of this protein at theplasma membrane in comparison with A Hall in a time-dependent manner. However, at 90 min of treatment, we observed 90% ofcytotoxicity, and further studies at this time were not evaluated. These results provide new insights about the NTBo mechanism ofaction and its possible use in the fight against breast cancer.