?Pro-apoptotic effect of amniotic membrane conditioned medium in hepatocarcinoma cells?

LUCIANO PÉREZ; RODRIGO RIEDEL; ANTONIO PÉREZ-PÉREZ; MARIANA JAIME; VICTOR SÁNCHEZ MARGALET; CECILIA LAURA VARONE; JULIETA LORENA MAYMÓ

Congreso; IX SLIMP-Latin American Society for Materno Fetal Interaction and Placenta.; 2022

SLIMP-Latin American Society for Materno Fetal Interaction and Placenta.

Objectives: The placenta and fetal membranes have recently been proposed as an important stem cells source for regenerative medicine. Stem cells derived from amniotic membrane offer considerable advantages over other stem cells because of the ease of collection, their low immunogenicity, and minimal ethical and legal barriers are associated with their use. Amniotic epithelial cells isolated from the amnion express embryonic stem cells markers and are pluripotent. Are Not only amnion-derived stem cells applicable in regenerative medicine, but also have antitumoral properties. Hepatic failure is one of the major causes of morbidity and mortality. In particular, hepatocarcinoma is the fifth leading cause of death worldwide. A few studies have demonstrated the antitumoral effects of the amniotic membrane and their cells, but little is known about the molecular and cellular mechanisms involved. The aim of this work was to analyze the expression of some important pro and antiapoptotic proteins, in hepatocarcinoma cells treated with amniotic membrane conditioned medium (AM-CM).Methods: Previous results showed that AM-CM inhibits the proliferation of HepG2 and HuH-7 cells. We have analyzed the expression of the proapoptotic proteins Bax, Bad and Bak and the antiapoptotic proteins Bcl-2, Bcl-XL and Mcl-1, by qRT-PCR and Western blot, in HepG2 and HuH-7 hepatocarcinoma cells, treated with AM-CM.Results: We observed a significant increment in proapoptotic mRNAs and proteins expression and a decrease in antiapoptotic genes and proteins expression, after 24 and 72 h of treatment with AM-CM. Moreover, AM-CM treatment increases the presence of apoptotic nuclei, measured by DAPI staining.Conclusion: Our results position amnion-derived stem cells as emerging candidates in anticancer therapy.