Funguemia and yeasts catheter colonization in hospitalized pediatric patients from northeast Argentina

GIUSIANO G; MANGIATERRA M; ROJAS F; GÓMEZ V; DIAZ MC

Santiago, CHILE

Congreso; 3rd. World Congress of Pediatric Infectious Diseases - WSPID; 2002

Yeast infection is an increasing problem in hospitalized children, particularly in neonatal (NCU) and intensive care units (ICU). Objectives: To determine: a) incidence of fungemia and yeast catheter colonization, b) characterization of yeast species  and  antifungal susceptibility profile.  Material and Method : From  February 1999 - 2002,  9465 blood samples were studied by lisis centrifugation and 909 catheters by Maki and Cleri methods in pediatric patients. Yeast identification was performed by Kreger van-Rij and Api ID 32C (bioMérieux) methods. Antifungal susceptibility was performed by the NCCLS micromethod M27A. Results: From 757 blood cultures positives, 88 (11,62%) were yeasts. From 200 catheters culture positives, 64 (32%) were yeasts. Of 88 funguemia, 31 belonged to NCU, 42 to ICU and 15 to cancer patients (CP). Of 64 yeast catheters colonization, 20 were from NCU, 37 from ICU and 7 from CP. Bloodstreams in ICU showed Candida albicans(C. albicans) (44,4%) and C. tropicalis (36,1%). Catheters C. albicans (45,9). C albicans 11/88 (36%) and C. parapsilosis 11/88 (36%) were the most  frequent species isolated  from neonate’s blood stream and C. parapsilosis 32/64 (50%)  from  catheters. Trichosporon cutaneum, C. krusei, C. glabrata and others Candida spp. were isolated from blood  and Malassezia species from catheters. C. parapsilosis was the predominant species (55,4%) among CP. All the isolates were susceptible to amphotericin B. C. albicans 3/49 (6,7%), C. tropicalis 3/31 (8,3%) and C. glabrata 3/6  were resistant to fluconazole. C. krusei (2/2 ) were resistant to fluconazole and itraconazole.  Conclusion: These data show the emergence of yeasts different from C. albicans as a cause of fungemia and catheter colonization.