CONICET | Buscador de Institutos y Recursos Humanos

p { margin-bottom: 0.21cm; } The knowledge of BR is very important for the treatment of bone pathologies and its evaluation. There are different methods that measure BR: histomorphometry, radioisotopes and bone markers, such as Dpd, CTX and osteocalcin. Bone markers are practical but very expensive and not always the assumptions made are correct. BR and formation are coupled in a variety of situations, therefore bone remodeling could be measured with one bone marker. Dpd appears as the most suitable technique: is measured in urine, has a lower cost than the others and two kits are available: ELISA and RIA. The former is overpriced but requires more afordable equipment and less skillful staff.RIA requires authorized laboratories and personnel but they are less expensive. The aim of this study was to compare two kits of urinary Dpd determination: ELISA and RIA, and to validate it in biological models with modified bone remodeling. Both kits were used to measured samples of rats from different ages, with increased BR (by ovariectomy, by hypocalcic diet (OVX+H) and by partial nephrectomy) and reduced BR (by treatment with zoledronate and by parathyroidectomy (PX)). Bone remodeling was confirmed by fluoride bone uptake and static histomorphometry.Dpd measured values (nmol/mmol creatinine) by RIA were as expected for each model. A significant linear regression was found in ELISA respect to RIA: r2=0.60, slope=0.59±0.06, p<0.005. Values obtained by ELISA of OVX+H and PX rats were not as expected. Conclusions: Values obtained by RIA were consistent with the biological models. ELISA values, in OVX+H and PX rats, were not as expected. ELISA could be underestimating Dpd measurements. RIA enables greater diferences between all the groups and is less expensive. These results indicate that it would not be advisable to compare values obtained by this two methods.

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