CONICET | Buscador de Institutos y Recursos Humanos

p { margin-bottom: 0.21cm; } Fluoride (F) enters the body through food, treatments for bone loss or prevention of dental caries. It has a positive effect on bone formation, but it can affect the oxidative stress.Previous reports has been performed usually with F concentrations higher than those found in vivo. The aim of this study was to evaluate the effect of F on OC with F concentrations similar to those in plasma after a dose for osteoporosis treatment or after the consumption of fluoridated water. OC was measured in slices of liver, kidney and muscle with F 0-100 μM. Sprague-Dawley rats were divided into three groups: Control (C), Treated with F (TF): 15 ppm F in drinking water for 30 days (model of caries prevention) and MFP: ovariectomized rats treated with monofluorophosphate 80 μmol/day for 60 days (model of osteoporosis treatment). After euthanasia, slices of tissues were successively exposed to 0, 10, 50 and 100 μM F. The presence of F decreased OC in liver and muscle in all gruops.The opposite was observed in kidney. OC consumption is shown as nmol/min.mg protein, mean±SEM. Muscle, 10, 50 μM not shown. *p<0.05 significant difference from 0 μM. Liver: C (0 μM: 15.7±2.5; 100 μM: 10.5±2.2*); TF (0 μM: 14.9±1.5; 100 μM: 9.4±1.1*); MFP (0 μM: 13.1±0.9; 100 μM: 10.2±0.8*). Kidney: C (0 μM: 18.2±1.2; 100 μM: 31.9±2.5*); TF (0 μM: 14.0±1.8; 100 μM: 29.0±5.2*); MFP (0 μM: 18.6±2.0; 100 μM: 32.0±3.0*). Transaminase and creatine kinase activity, creatinine clearance and histological study did not show significant changes in tissues at the beginning of the measurements. Conclusion: F produces changes in OC at concentrations comparable to those found in vivo. These results would indicate that the modification of oxidative stress could be due to changes in the production of reactive oxygen species at the level of the respiratory chain.

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