PARTICIPATION OF ADENYLATE CYCLASE ISOENZYMES, SOLUBLE AND MEMBRANE-ASSOCIATED, IN CRYOPRESERVED BOVINE SPERMATOZOA CAPACITATED WITH HEPARIN

FERNÁNDEZ S; CÓRDOBA M

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Jornada; XXIII Jornada Anual de la Sociedad Argentina de Biologia; 2021

Sociedad Argentina de Biologia

PARTICIPATION OF ADENYLATE CYCLASE ISOENZYMES, SOLUBLE AND MEMBRANE-ASSOCIATED, IN CRYOPRESERVED BOVINE SPERMATOZOA CAPACITATED WITH HEPARINFernández S1,3, Córdoba M1,2,31Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Buenos Aires, Argentina. 2CONICET-Universidad de Buenos Aires. Unidad Ejecutora de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina. 3Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Química Biológica. Buenos Aires, Argentina. mcordoba@fvet.uba.arSoluble and membrane-associated isoenzymes of adenylate cyclase are present in the intracellular signal system of sperm capacitation. It is interesting to determine which inducers and inhibitors can activate or block them to unchain this sperm process, since the way these enzymes modulate the signals is controversial. The objective of this study was to determine capacitation, viability, motility and mitochondrial activity of cryopreserved bovine spermatozoa treated with heparin and inhibitors of the soluble and membrane-associated adenylate cyclase enzymes. Heparin was used as a capacitation inducer, LRE-1 as a soluble adenylate cyclase inhibitor and 2,5-dideoxyadenosine as a membrane-associated adenylate cyclase inhibitor. Capacitation was evaluated by the chlorotetracycline epifluorescent technique and viability and membrane integrity by the trypan blue vital staining with interferential differential contrast. Sperm motility was evaluated by microscopy and analyzed with the ISAS-Prosier software. Mitochondrial membrane potential was measured using JC-1 fluorochrome. Data were analyzed by ANOVA and Tukey´s test (P