INTERGENERATIONAL EFFECTS OF MODERATE PATERNAL ALCOHOL INTAKE

BIROLO SOL CAMILA; STINSON MARCELO GABRIEL; FESSER ESTEFANÍA AYLÉN; CAMBIASSO MAITE YAEL

Encuentro; Latin American Society for Biomedical Research on Alcoholism (LASBRA); 2020

SAIC-SAI-SAFIS

Previously, we observed that male alcohol intake affects sperm biochemicalparameters and DNA integrity. The resultingprogeny was underweight during their firstweeks of life, and overweight in theiradulthood, and showed altered spleen cell populations. Aim: To assess the effectsof moderate paternal alcohol consumption on different physical and behavioralparameters of its offspring (F1) development. Additionally, we screened forpotential modifications in the expression of candidate genesin the medial prefrontal cortex (mPFC) ofF1 mice. CF1 male mice were exposed (A) or not (C) to15% (v/v) ethanol in drinking water ad libitum for 15 days, followingwhich they were mated (1:1) with non-treated CF1 females.Two to 17 days old F1 mice weresubjected to developmental tests (surface righting,grasping reflex, negative geotaxis, limbs suspension, grip strength, cliffaversion, ambulation tests), and two to four monthsold F1 mice to behavioral tests (open field or OF,elevated plus maze, object context recognition test and social dominance tubetest). Brains from adult F1 mice were dissected, and those from A weighed less than those from C (p