INTERGENERATIONAL EFFECTS OF MODERATE PATERNAL ALCOHOL INTAKE

CAMBIASSO MAITE YAEL; ROMANATO MARINA; REY VALZACCHI GASTÓN; FONTANA, VANINA A; CALVO LUCRECIA; CALVO, JUAN CARLOS

Buenos Aires

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

SAIC

Assisted reproductive treatments (ART) bypass male infertility, removing most barriers to fertilization. However, there are cases inwhich these techniques fail due to idiopathic causes. In search ofpossible causes of infertility in couples, we studied the presenceof post-translational marks on the remaining histones in humanspermatozoa, as it has been found that they are involved in thefunctionality of the post-fertilization zygote and may contribute toembryonic development. The aim of the study was to evaluate therelationship between the remaining sperm histones and the successof ART. In this study, the presence of modified histones was analyzed by immunocytochemistry using antibodies against H3K4me3,H3K27me3, H3K9me2/3, H3K4me2, H3K9me and H4K12ac. Thepresence of these marks was studied in sperm samples from normozoospermic donors (n = 5) and from infertile patients undergoingassisted reproductive techniques (n = 13) and then, we correlatedthem with sperm parameters and ART success. A negative correlation was found between H3K4me3 and H3K27me3 modificationswith motility in donor sperm (p