Application of a proteomic approach to decipher biological processes and functional organellar components for dual-cell photosynthesis in Setaria viridi

CALACE, P.; MARGARIT, E.; TONETTI, T.; FIGUEROA, C.; ANDREO, C.S.; GERRARD WHEELER, M.; SAIGO, M.

Santa Fe

Congreso; RAFV 2021 - XXXIII Argentinian meeting of Plant Physiology; 2021

SAFV

The C4 photosynthesis is typically characterized by the spatial compartmentation of the photosynthetic reactions into mesophyll (M) and bundle sheath (BS) cells. The initial carbon fixation gives rise to C4 acids within M cells, which are transported to the BS cells. There, the C4 acids are decarboxylated so that the resulting CO2 is incorporated into the Calvin cycle. This work is focused on the study of Setaria viridis, a C4 model close relative of several major feed and bioenergy grasses. According to the peptide identification and quantification by mass spectrometry, 1,376 proteins were identified in M and BS total protein samples. The statistical analysis showed that 489 proteins were more abundant in M and 336 in BS, which would support different metabolic scenarios. Gene ontology analysis and subcellular location prediction evidenced significantly different proteomes of chloroplasts, mitochondria and peroxisomes in M and BS. This can be related to the different functions of the organelles in M and BS compartments and also shows metabolic strategies to insulate the C4 core to avoid the leakage of intermediates. In this presentation we describe a useful pipeline to manage proteomic data that uncovered novel lines to keep studying and expanding the knowledge on this topic.