EARLY PROTEOME PROFILE OF SOYBEAN EMBRYONIC AXES GERMINATION.

YORDÁN. E.; PEROTTI, V.E.; MARGARIT, E.; MARTINEZ, M.; GOSPARINI, C.; MONTECCHIARINI, M.L.

Santa Fe

Congreso; RAFV 2021 - XXXIII Argentinian meeting of Plant Physiology; 2021

SAFV

Seed germination implies restarting of embryonic axis growth, with cell expansion defining radicle protrusion. Isolate soybean embryonic axes (Ax) [Glycine max (L. Merr)] start expansion from 10 h, completing germination at 12 h of water incubation. The present work aimed to evaluate the early proteome profile promoting Ax germination. One hundred Ax were incubated for 9 h in distilled water at 27 ± 1 ºC and in the dark. Samples of 1 g Ax were powder in liquid N2, homogenized in 5 mM NaAc pH 4.6 buffers containing Sucrose gradients (0.4?1) M, followed by sequential extraction in 0,2 M CaCl2 (Ca), 2 M LiCl (Li) and Urea buffer (U) and final precipitation in TCA 100 %. A total of 40, 103 and 946 unique proteins with at least two high confidence peptides (Uniprot and Phytozome), of which 5, 33 and 888 were exclusively identified in Ca, Li and U. The total exclusive proteins localized (TargetP) as Secretory (118), Mitochondrial (161) Chloroplastic (156) and unknown (491). The gene ontology classification (AgriGO) for orthologue proteins of Arabidopsis thaliana showed enrichment in translation and gene expression processes, catabolism and energy, and embryo development ending in seed dormancy. These results evidenced a great cellular activity associated with early events defining soybean embryonic axes germination.