Maintaining normal root morphology in Brugmansia candida transformed root cultures

PITTA ALVAREZ, SANDRA; GIUILIETTI, ANA MARÍA

Phytochemicals and Health

American Society of Plant Physiologists

Lugar: Maryland; Año: 1996; p. 326 - 329

Scopolamine and hyoscyamine are tropane alkaloids widely used in medicine as anticholinergic agents. The biosynthesis of these compounds takes place mainly in the roots of a large number of species of Solanaceae. Hairy root cultures are a valuable alternative for in vitro production of these alkaloids. Hairy root cultures of Brugmansia candida were established. B. candida transformed roots showed a strong tendency towards dedifferentiation. Different strategies were tested to improve this situation.