Study of Neurotoxic Intracellular Calcium Signalling Triggered by Amyloids

VILLALOBOS, C; CABALLERO, E; SANZ BLASCO S; NÚÑEZ, L

Methods in Molecular Biology

Humana Press

Año: 2012; p. 289 - 302

Neurotoxicity in Alzheimer?s disease (AD) is associated to dishomeostasis of intracellular Ca2+ induced by amyloid β peptide (Aβ) species. Understanding of the effects of Aβ on intracellular Ca2+ homeostasis requires preparation of the different Aβ assemblies including oligomers and fibrils and the testing of their effects on cytosolic and mitochondrial Ca2+ in neurons. Procedures for cerebellar granule cell culture, preparation of Aβ species as well as fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca2+ in neurons are described.