INVESTIGADORES
SEIJO Jose Guillermo
capítulos de libros
Título:
A Genomic characterization of the chili peppers (Capsicum, Solanaceae) germplasm by classical and molecular cytogenetics.
Autor/es:
E A. MOSCONE.; SCALDAFERRO, M. A.; GRABIELE M; ROMERO, M; DEBAT, H; J. G. SEIJO; J. R. DAVIÑA; ACOSTA, M. C.; BARBOZA, G. E.; DA DUCCASE
Libro:
Physical Mapping Technologies for the Identification and Characterization of Mutated Genes Contributing to Crop Quality
Editorial:
International Atomic Energy Agency
Referencias:
Año: 2011; p. 97 - 105
Resumen:
Abstract
Within the framework of the IAEA coordinated research project entitled ?Physical mapping technologies for the
identification and characterization of mutated genes contributing to crop quality? we carried out genomic
characterization of wild and cultivated samples of chilli peppers (genus Capsicum) by classical chromosome
staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH).
For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e.
chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich
regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. The
heterochromatin could be characterized in respect to type, amount and distribution in the different accessions
examined. On the other hand, the number and position of active NORs could be determined. Using FISH,
different DNA probes were used in order to map specific sequences in the chromosomes, i.e. 45S and 5S rDNA,
telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done, we
present the results obtained on a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH).
For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e.
chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich
regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. The
heterochromatin could be characterized in respect to type, amount and distribution in the different accessions
examined. On the other hand, the number and position of active NORs could be determined. Using FISH,
different DNA probes were used in order to map specific sequences in the chromosomes, i.e. 45S and 5S rDNA,
telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done, we
present the results obtained on a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Capsicum) by classical chromosome
staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH).
For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e.
chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich
regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. The
heterochromatin could be characterized in respect to type, amount and distribution in the different accessions
examined. On the other hand, the number and position of active NORs could be determined. Using FISH,
different DNA probes were used in order to map specific sequences in the chromosomes, i.e. 45S and 5S rDNA,
telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done, we
present the results obtained on a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Within the framework of the IAEA coordinated research project entitled ?Physical mapping technologies for the
identification and characterization of mutated genes contributing to crop quality? we carried out genomic
characterization of wild and cultivated samples of chilli peppers (genus Capsicum) by classical chromosome
staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH).
For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e.
chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich
regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. The
heterochromatin could be characterized in respect to type, amount and distribution in the different accessions
examined. On the other hand, the number and position of active NORs could be determined. Using FISH,
different DNA probes were used in order to map specific sequences in the chromosomes, i.e. 45S and 5S rDNA,
telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done, we
present the results obtained on a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH).
For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e.
chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich
regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. The
heterochromatin could be characterized in respect to type, amount and distribution in the different accessions
examined. On the other hand, the number and position of active NORs could be determined. Using FISH,
different DNA probes were used in order to map specific sequences in the chromosomes, i.e. 45S and 5S rDNA,
telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done, we
present the results obtained on a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Capsicum) by classical chromosome
staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH).
For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e.
chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich
regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. The
heterochromatin could be characterized in respect to type, amount and distribution in the different accessions
examined. On the other hand, the number and position of active NORs could be determined. Using FISH,
different DNA probes were used in order to map specific sequences in the chromosomes, i.e. 45S and 5S rDNA,
telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done, we
present the results obtained on a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Within the framework of the IAEA coordinated research project entitled ?Physical mapping technologies for the
identification and characterization of mutated genes contributing to crop quality? we carried out genomic
characterization of wild and cultivated samples of chilli peppers (genus Capsicum) by classical chromosome
staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH).
For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e.
chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich
regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. The
heterochromatin could be characterized in respect to type, amount and distribution in the different accessions
examined. On the other hand, the number and position of active NORs could be determined. Using FISH,
different DNA probes were used in order to map specific sequences in the chromosomes, i.e. 45S and 5S rDNA,
telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done, we
present the results obtained on a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH).
For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e.
chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich
regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. The
heterochromatin could be characterized in respect to type, amount and distribution in the different accessions
examined. On the other hand, the number and position of active NORs could be determined. Using FISH,
different DNA probes were used in order to map specific sequences in the chromosomes, i.e. 45S and 5S rDNA,
telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done, we
present the results obtained on a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Capsicum) by classical chromosome
staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH).
For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e.
chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich
regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. The
heterochromatin could be characterized in respect to type, amount and distribution in the different accessions
examined. On the other hand, the number and position of active NORs could be determined. Using FISH,
different DNA probes were used in order to map specific sequences in the chromosomes, i.e. 45S and 5S rDNA,
telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done, we
present the results obtained on a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Capsicum annuum var. annum (cultivar NMCA 10272), the most
broadly exploited cultivar of chilli pepper. The results allowed us to characterize the Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.
Capsicum species and
accessions and the possible evolutionary pathways for chilli peppers was deduced based on the available
cytogenetic data. It is worth mentioning that the research work done under this CRP is part of work being done
within an exsting network of chilli pepper research of this important plant group utilized by man and among one
of the first cultivated plants in the history of humanity.