Transport Studies in Plasma Membrane Vesicles Isolated from Renal Cortex.

GISELA DI GIUSTO; ADRIANA M. TORRES

Experimental Surgical Models in the Laboratory Rat

CRC PRESS-TAYLOR & FRANCIS GROUP

Lugar: Boca Ratón, FL, USA.; Año: 2009; p. 189 - 194

Transcellular transport in renal tubules involves the movement of solutes between three compartments: luminal, intracellular, and peritubular. Two barriers separate them: the apical and the basolateral plasma membranes. The two membranes are different in almost every respect, such as morphology, enzyme content, protein–lipid–carbohydrate composition, hormone receptors, and transport properties. These differences are emphasized in the procedures for membrane separation. This chapter contains a full description of the preparation of basolateral membrane vesicles and brush border membrane vesicles from rat kidney cortex. Furthermore, it includes an example of transport studies in these membrane vesicles using the organic anion p-aminohippurate and the experimental results obtained in male and female rats.