Murine colonization model by Campylobacter coli DSPV458

RUIZ, M. J.; SOTO, L. P.; SIRINI, N. E.; WERNING, M. L.; OLIVERO, C. R.; ZIMMERMANN, J. A.; ZBRUN, M. V.; ACOSTA, F. F.; SIGNORINI, M. L.; FRIZZO, L. S.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2022 vol. 132 p. 1457 - 1466

1364-5072

Aims: To generate a murine experimental model of colonization by Campylobactercoli DSPV458. Methods and Results: Twelve adult Balb/cCmedc female mice were housed in atreated group (T-G) and a control group (C-G) for 4 weeks. Both experimental groupsreceived antibiotics for 5 days during the first week. The T-G was administered with6.68log10CFU of C. coli DSPV458 by oesophageal gavage. Necropsies were performedweekly to evaluate translocation and intestinal colonization in the spleen and liverand in the ileum and cecum respectively. Samples were cultured to quantify intestinal microbiota members. Faeces were cultured weekly for a C. coli DSPV458 count.Campylobacter coli DSPV458 was isolated from all the inoculated mice. The recovered level of C. coli DSPV458 was, on average, 6.9 log10CFUg−1, 8.0 log10CFUg−1 and1.6 log10CFUg−1 in faeces, cecum and ileum respectively. Colonization by C. coliDSPV458 does not alter the normal clinical and physiological status.Conclusions: Campylobacter coli DSPV458 does not have an invasive capacity, andthe model is suitable for evaluating strategies to reduce intestinal loads.Significance and Impact of Study: Farm animals have an important impact onthermotolerant Campylobacter transmission to humans. Extremely few colonizationmodels by C. coli have been reported to date. In food-producing animals, infection ismild or absent and thermotolerant Campylobacter colonize the intestines of animals.Colonization models are specific models that do not cause infection as they do notgenerally result in diarrhoea or other signs of disease. Therefore, this model willallow to evaluate the evolution of colonization by thermotolerant Campylobacter andthe alternative tools development to antibiotics that limit their colonization in foodproducing animals.