Abstract: KERATINS SEQUENCE IDENTIFICATION IN LLAMA GENOME

APICHELA, S; ROLDAN OLARTE, MARIELA; ROMERO, S; MICELI, DC

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2010 vol. 34 p. 180 - 180

0327-9545

The production of fabrics from llama fiber represents na important economic activity for the Andean communities of Argentina. The quality ADN possibilities of industrialization of the fiber are influenced by its physio-mechanical characteristics. In sheep it is known that these properties would be related to the type of keratins in the wool. Five types of keratin intermediate filaments are known. Type I and type II keratins are present in wool and hair. There is no avaible information about keratins gene sequences of South Americans camelids in biological databases. Our objective was to study some sequences of llama in order to relate the gene expression of fiber quality parameters. DNA was isolated from anticoagulated blood samples of llama. Primers were designed from sequences of the human keratins genes that are expressed in the hair follicle, specifically type I keratins: K32 and K 34. The llama DNA templatesobtained were used in the PCR reaction. Human DNA was used as a positive control. A 1200 pb Fragment was obtained with the K32 primers, whose size corresponds to the one observed in the control, and two fragments of approximately 800pb were obtained with K34 primers, larger than the control. The three amplified products were sequenced. Knowledge of these sequences will enable the study of keratin expression in the llama hair follicle.