CONTRIBUTION OF AQUAPORINS IN THE TRANSAMNIOTIC WATER FLUX

DI PAOLA MAURICIO; SIERRA MATÍAS; FERNANDEZ NAZARENA; IBARRA CRISTINA; DAMIANO ALICIA

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2021

0006-291X

We explored the contribution of each aquaporin(AQP) expressed in human amnion in the transcellular water flux across thehuman amnion. Human amnion was placed between two lucite chambers and net watertransport (Jw) was recorded by applying a hydrostatic (7 cm H2O) and an osmotic(40 mOsm PEG 8000) pressure gradients. The hydrostatic (Phydr) and osmotic(POsm) permeabilities were calculated before and after the blocking of AQPs.Phdr showed no significant difference after the blocking of AQPs, while POsmwas dramatically reduced. Interestingly, we also found that the blocking ofAQP1 produced the highest decrease of POsm (80±1%). Our results stronglysuggested that AQP1 seems to contribute more to the maintenance of AF volume homeostasis @font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:swiss;mso-font-pitch:variable;mso-font-signature:-520082689 -1073697537 9 0 511 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-fareast-language:EN-US;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-fareast-language:EN-US;}div.WordSection1{page:WordSection1;}